23 Questions 17 Answers 0 Followers
Questions related from Ping Kwan
Dear colleagues, I was wondering whether there are any ways or any softwares that I can use to analysis the muscle cross sectional area in my H&E histology images? I tried to use ImageJ...
04 April 2016 6,843 9 View
If I am going to make a guess between the potassium channel and the chlroide channel by the following evidence: "hyperpolarization of pyramidal neurons and suppresses action potential firing in...
10 October 2015 623 0 View
What is the intracellular distribution of Matrix Metalloproteinase (MMP)-9 in fast motor neurons (if possible, which organelle)? Do they found in the axon terminal? Additionally, do microglia...
09 September 2015 6,618 3 View
Dear colleagues, I was wondering if anyone know the specific or unique molecular markers for the following ANS fibers (I would like to do some immunohistology to distinguish between the non-ANS...
09 September 2015 6,496 1 View
I am recently planning to do some stereotaxic injections to some mice brain nuclei. Due to the various choices (in particular the G number) of Hamilton needles, I was wondering which one would be...
03 March 2015 5,867 4 View
I have a question regarding the use of control as follows: Although it is quite usual to setup 2 groups of animals (one for experiment, and the other for control), there is some paper/studies...
02 February 2015 2,891 8 View
I am planning to do a stereotaxic injection to the medial forebrain bundle (MFB) of mice. I have tried AP:-1.2; ML:-1.1; DV:-5.0mm, but this does not seem to work. I was wondering whether there is...
02 February 2015 9,938 7 View
In the literature, there is often a description saying that ''an animal weight X to Y grams/pounds was used''. I was wondering how do the author know the best weight range of their experimental...
02 February 2015 2,445 7 View
As M1 and M2 lie very close to each other, if I am going to do an implant to gather signals from only M1 neurons, is there any established way for me to locate accurately the M1 or M2? The...
01 January 2015 5,549 1 View
If I am going to do an in vivo patch clamp on mice M1 neuron (primary motor cortex) only, is there a way to identify/confirm the area? To the best of my knowledge, M1 is close to M2 and these 2...
01 January 2015 5,554 4 View
After the fixation with 4% paraformaldehyde (PFA), I assume that everything that is fixable by PFA will be preserved while those did not will lost much of the attention from the investigator (may...
01 January 2015 7,747 6 View
In regard to the preparation of acute brain slices, animals are anaesthetised, confirmed areflexia, and then decapitated. In my case, if I am going to study the hippocampus, then only the regions...
12 December 2014 1,453 0 View
Assume that I have a liquid (ddH2O) has a mixture of unknown ions (in different concentrations), I was wondering that if there is an assay for me to demonstrate the ion composition pattern in this...
12 December 2014 6,566 7 View
I am currently working with flowering plants, as I need to quantify the content of chlorophyll (particular subtype, e.g. chlorophyll a) in a specific leaf after its extraction, I was wondering...
12 December 2014 4,717 13 View
I was wondering when did APOE started to classify into E2, E3 and E4 isoforms and who created/provided the definition of these different isoforms. If corresponding paper can be provided for...
08 August 2014 7,351 0 View
I was wondering how should I do my statistical test with the following experiment: Reaction components: A + B (when mixed, the reaction rate is recorded) Variables: Temperature, pH, sunlight...
08 August 2014 6,755 3 View
After a search on the NCBI database, I found only the record for APOE precursor, APOE2 and APOE3 sequences. I am looking for any information regarding the E4 isoform (i.e. APOE4) sequence and if...
07 July 2014 7,377 6 View
After the first (agarose or SDS-PAG) electrophoresis, if I run the same gel again with an-overtime so that everything in the gel will now in theory come off the gel (i.e. electro-rinsing), then...
03 March 2014 3,571 11 View
If watercrystal damage happened in my whole brain samples, would there be any significant differences in the result of my immunoblotting between step A and step B (which are as follows)? Step A:...
02 February 2014 882 2 View
As I would like to isolate different cell types from my frozen-damaged brain tissues, is it still possible to use immuno-relevant methods to do the isolation/extraction of cells from the whole...
02 February 2014 3,884 7 View
What is the best extraction protocol if I would like to check the levels of the following proteins: Akt, β-catenin, HIF-1α, GLUT1, GSK-3β, p-GSK-3β, Tau, p-Tau, AMPH1, Aβ40 and Aβ42. Thank you...
02 February 2014 5,652 4 View
I was wondering if someone can tell me the character of different protein fractions extracted from different buffers, e.g. the Tris-, RAB-, RIPA-, FA-soluble, sarkosyl-insoluble fractions? I was...
02 February 2014 8,790 4 View
(In addition to the commonly used "head ring")
03 March 2013 2,247 1 View
