I would highly recommend to check the coordinates with dye injections and keep mouse strains, weight and age constant. Always measure your Bregma-Lambda distance and check for flat head. We found some small variations in the same strain but different breeders as well as between laboratories. The coordinates that Moises reports we have used repeatedly and they work well in our hands, with male C57/Bl6 mice. Pay attention to the post-operative care as many mice will lose a lot of weight starting 2-3 days after surgery and some people have reported high mortality rates.

Hello,

I have only used 6-OHDA in mice injecting it in the striatum, but I have found some articles (e.g. http://onlinelibrary.wiley.com/doi/10.1111/ejn.12150/full) where they lesion the MFB using these coordinates:

(from bregma): AP, −1.2 mm, ML, −1.2 mm; and DV, −4.75 mm (from dura)

Good luck!

http://onlinelibrary.wiley.com/doi/10.1111/ejn.12150/full

I would highly recommend to check the coordinates with dye injections and keep mouse strains, weight and age constant. Always measure your Bregma-Lambda distance and check for flat head. We found some small variations in the same strain but different breeders as well as between laboratories. The coordinates that Moises reports we have used repeatedly and they work well in our hands, with male C57/Bl6 mice. Pay attention to the post-operative care as many mice will lose a lot of weight starting 2-3 days after surgery and some people have reported high mortality rates.

i won't lie that i have locate the MFB before but i believe you will find this material very useful although it is the rat brain in stereotaxis coordinate by paxinos and WATSON.

i hope you find it useful

AP -1.5 ML -1.5 DV -5.0 with this coordinates you should hit the mfb.

Alessandro 

Hi,

I agree with Andreas!

Faranak

Look for the coordinates in the brain atlas or try injecting a dye to confirm.