Best mounting medium for plant-tissue coloured with WGA-AF488 staining?

Hi everybody, I am preparing some plant tissue microscope slides, and I am doing a specific staining for fungi coloration (WGA-AF488 in PBS buffer pH 7.2). I was wondering what would have been...

26 November 2020 6,118 3 View

How to model a graphite-bearing rock using Perplex (pseudosections)?

Dear all, I am struggling to model a rock which contains the assemblage K-white mica + Na-white mica + chlorite + quartz + graphite + rutile. I have followed step by step the tutorial available on...

22 November 2020 2,387 2 View

How can I convert emuOe/g in A/m?

From a research I got the saturation values of an MH curve in "emuOe/g" but I need them to be in A/m. My doubt here is is the data in "(emu/g) * Oe" or that "emu" means only "electromagnetic...

30 June 2020 2,356 8 View

Does anybody used Assess 2.0. Image Analysis Software?

I analized six sets of 26 leaves using the same process with the Automatic Panel. I used the same thresholds and the same calibration for all of them. In only one of the sets the data are...

15 January 2018 3,627 2 View

Can anyone help with optimized crystallographic files of MAPbl(3-x)Clx, MAPbCl3 ? (Mixed Halide Perovskite)

20 July 2014 7,651 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Have you ever experiencing air vent trouble in fermentation process?

Dear all, My research group is currently working with a 12-litre fermenter. Somehow, if we do the fermentation for more than 20 hours, the process will be more prone to contamination. Our current...

23 February 2021 1,908 2 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View

Who are the possible experts I can reach out to regarding the reseach topic: Activated Carbon as Water Filter?

Hi! Me and my team mates are currently new in doing a research study. We do not have any previous experiences in research. And we just wanted to know what type of experts (the field they in) can...

19 February 2021 9,596 4 View

Why my protein band in SDS-PAGE if heating the sample becomes much lighter compared to not heating the sample?

I ran a SDS-PAGE of my sample ovalbumin in histidine buffer in 2x Laemmli buffer without heating and it got nice visible bands. However, when I heat the sample at 77C in 2x laemmli buffer for 5...

15 February 2021 3,080 3 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View