Best mounting medium for plant-tissue coloured with WGA-AF488 staining?

Hi everybody, I am preparing some plant tissue microscope slides, and I am doing a specific staining for fungi coloration (WGA-AF488 in PBS buffer pH 7.2). I was wondering what would have been...

26 November 2020 6,118 3 View

How to model a graphite-bearing rock using Perplex (pseudosections)?

Dear all, I am struggling to model a rock which contains the assemblage K-white mica + Na-white mica + chlorite + quartz + graphite + rutile. I have followed step by step the tutorial available on...

22 November 2020 2,387 2 View

How can I convert emuOe/g in A/m?

From a research I got the saturation values of an MH curve in "emuOe/g" but I need them to be in A/m. My doubt here is is the data in "(emu/g) * Oe" or that "emu" means only "electromagnetic...

30 June 2020 2,356 8 View

How to stop enzymatic reaction without hurting bacteria?

Hello everyone. I need to test a reaction mix against bacteria, to state its inhibitory potency: how can I stop the reaction? I tried filtration using MicroCons, but part of the product is lost,...

09 January 2020 9,909 3 View

Does anybody used Assess 2.0. Image Analysis Software?

I analized six sets of 26 leaves using the same process with the Automatic Panel. I used the same thresholds and the same calibration for all of them. In only one of the sets the data are...

15 January 2018 3,627 2 View

Can anyone help with optimized crystallographic files of MAPbl(3-x)Clx, MAPbCl3 ? (Mixed Halide Perovskite)

20 July 2014 7,651 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View