Best mounting medium for plant-tissue coloured with WGA-AF488 staining?

Hi everybody, I am preparing some plant tissue microscope slides, and I am doing a specific staining for fungi coloration (WGA-AF488 in PBS buffer pH 7.2). I was wondering what would have been...

26 November 2020 6,118 3 View

How to model a graphite-bearing rock using Perplex (pseudosections)?

Dear all, I am struggling to model a rock which contains the assemblage K-white mica + Na-white mica + chlorite + quartz + graphite + rutile. I have followed step by step the tutorial available on...

22 November 2020 2,387 2 View

How can I convert emuOe/g in A/m?

From a research I got the saturation values of an MH curve in "emuOe/g" but I need them to be in A/m. My doubt here is is the data in "(emu/g) * Oe" or that "emu" means only "electromagnetic...

30 June 2020 2,356 8 View

How to stop enzymatic reaction without hurting bacteria?

Hello everyone. I need to test a reaction mix against bacteria, to state its inhibitory potency: how can I stop the reaction? I tried filtration using MicroCons, but part of the product is lost,...

09 January 2020 9,909 3 View

Does anybody used Assess 2.0. Image Analysis Software?

I analized six sets of 26 leaves using the same process with the Automatic Panel. I used the same thresholds and the same calibration for all of them. In only one of the sets the data are...

15 January 2018 3,627 2 View

Can anyone help with optimized crystallographic files of MAPbl(3-x)Clx, MAPbCl3 ? (Mixed Halide Perovskite)

20 July 2014 7,651 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Can I use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography?

Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...

22 February 2021 730 2 View

What software is capable of opening a file with the extension ".cad" ?

It seems like quick cad was the software but had been discontinued. I tried with other Autodesk software such as fusion 360, AutoCAD. I also tried with QCAD. Please let me know if you know of any...

22 February 2021 9,634 3 View

How to seperate PEG-4000 from aqueuse phase ?

Hello everyone , Please tell me if It possible to seperat POlyethelyn glycol -4000 from an aqueuse media by other ways rather heating . I have tried salting out by electrolyte solution to...

22 February 2021 7,285 5 View

What is the best technique for combining images?

Hi, Sorry I don't have much experience in image processing. In my research, I need to combine 2 grayscale images(like attached images) into one! I came across the image fusion process. In the...

16 February 2021 7,317 7 View

Does the property of GFP affect a fusion protein's localization?

Hello, I am planning to insert GOI between CaMV 35 promoter and mGFP in pCambia1302 to produce a fusion protein and see subcellular localization of the protein. But the vector says it's membrane...

15 February 2021 4,353 3 View

Buillding 3D Model Fussion Protein with Modeller?

Hello May i ask you about using Modeller for making 3D model fussion protein. I want to build a fusion/chimera protein structure which is including 2 different protein and then evaluate quality...

09 February 2021 9,369 2 View

In what type of container can we put a 200 degree Celsius poly alkylene glycol? what should be the material of the container?

I am having a 200-degree Celcius heated poly alkylene glycol now I want to know in which type of container should I put that heated glycol or what should be the material of the container?

08 February 2021 6,819 1 View

Does the power law behavior can be approximated with Gaussians?

In epidemiology, earthquakes, tokamak disruptions etc., there is possibility of approximations with the sequence of Gaussians and with the appropriate risks ( see my papers in Journal of Fusion...

26 January 2021 5,411 5 View