I tried to immobilize liposomes on raw mica, mica or glass functionalized by BSA or polylysine, but I observed completely different behaviours in their adsorption and stability depending on their composition. I also read papers which assert that irregular surfaces favour liposome stability. Have you directly experienced a general method to immobilize liposomes and maintain it stable? It's quite simple for commercial mixtures of lipids (probably they contain some components which favour their stability and their adsorption) but I would be interested to immobilize liposomes made by very simple and basic composition. Is there some lipid which favour the adsorption at best of your knowledge? Thanks for help
Dear Samuele,
I do not know your basic lipid composition but, since the surfaces you have used are highly hydrophilic and negatively charged, it is generally not easy to get intact (phosphatidyl choline - PC based) liposomes on that type of surfaces. In that case, please try with a negatively charged lipid type, e.g. PS (phosphatidyl -serine) or cholesterol up to 50 %, in which increase the rigidity of the bilayer of liposomes that will prevent the disruption.
Further, to prevent the probable exposure of the liposomes to air during experimetns, liposomes should be remained fully hydrated.
I'm also interested in this matter. I know that some AFM studies can be made without drying the sample which could disrupt liposomes since they are not exactly rigid particles. I would recommend this paper: Article AFM, ESEM, TEM, and CLSM in liposomal characterization: a co...
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