Hi everyone,
I have a solution ( containing fruit powder, media and probiotics)
I need to calculate the growth of particular probiotics in my solution, to see if the fruit has prebiotic activity. thats why I need to sterilize my solution first before adding the bacteria to it. but as it will have fruit I dont know if I can sterilize it with the media in autoclave or not. As high temperature may affect its prebiotic activity. Your guidance is so appreciated specially about common sterilization methods as we dont have much facilities.
Hi
You can do filtration method with 0.44 or 0.22 micrometer filter.
Show your fruit structure to a chemist for autoclave and so you check it yourself.
Hello you should have autoclaved the solution with fruit powder and media. The add prebiotics after the autoclave. But since you have the solution already i will recommend you to autoclave at lower temperature.
Dear Dana,
Then autoclave the solutions separately. You can then vary the temperature to avoid caramilization. Since you know the temperature at which caramillization occur.
You have to standardise the method of sterilisation of fruit powder at first. You may try the following methods:
1. U.V light steriisation
2. Filtration - as stated previously
3. Autoclave - as stated previously
4. Pasteurisation at 70 degree centigrade for 10 minutes
5. Boiling.
The effect of those procedures may be compared.
IT WILL ITSELF BE A VERY GOOD RESEARCH.
We add the probiotic after we pasteurized and the media were cooled down
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