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Questions related from Dana Perv
Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...
03 March 2018 1,363 4 View
Dear all. I have access to ankom fiber analyzer which is mostly useful for measuring ADF, NDF and crude fiber for animal food. I want to do preliminary test to see if my fruits have prebiotic...
02 February 2018 6,452 3 View
Hi everyone, I need to measure short chain fatty acids from my experiment, but my problem is limited volume of sample I have during the experiment. I know for HPLC of short chain fatty acid 20...
12 December 2017 8,493 2 View
Dear Friends, I want to measure prebiotic activity of some samples (by comparing growth of 3 bacteria (2 prbiotics and one E.coli ) on some samples to see if the samples are encouraging for good...
12 December 2017 2,374 1 View
Hi everyone, I received a Bifidobacterium lactis in freeze dried form, I revived it in media and I took its growth curv. Also, I did streak plating to have the colonies as back up and put in the...
12 December 2017 9,196 0 View
Hi everyone, I have a solution ( containing fruit powder, media and probiotics) I need to calculate the growth of particular probiotics in my solution, to see if the fruit has prebiotic activity....
11 November 2017 2,363 11 View
Hi everyone, I want to measure prebiotic activity of a fruit powder ( using fecal microbiota), I am wondering if I measure short chain fatty acids at the end of the test , how can I know that...
08 August 2017 2,134 5 View
I have made methanolic extract of a specific flower to use as antioxidant in frying, but the moment I added it to the hot oil, it burned, while many papers have used the extract in temperature of...
07 July 2017 8,955 4 View
Confused about choosing which protocol among many protocols for different assays and this time about DPPH assay! please suggest me any good and well known DPPH protocol. And in general if you know...
07 July 2016 8,891 1 View
Different literatures read spectrophotometer in different time, I am very confused how and when to read the numbers of spectrophotometer and how to zero it. Please let me know if you can clarify...
07 July 2016 6,243 4 View
Hi, I did the total flavonoid test but the standard solution curve does not seem good enough (R square 77 percent, Can I run just the standard sololution again? Or do I have to do the whole test...
07 July 2016 6,761 3 View
Hi, In Folin Ciocalteu test for measuring total phenolic content , once I run the test with 50 % dilution and another time with 1/10 dilution of sample as well as standard. In another word, in...
07 July 2016 5,438 7 View
Hi, there are many protocols in different papers but it is hard to know which one would be reliable. I am looking for measuring Total phenol content, Total Flavonoid Content and Antioxidant...
06 June 2016 2,397 6 View
