What is the pH to reach in measuring titratable acidity?

Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...

02 March 2018 1,265 4 View

Can I use ADF NDF crude fiber values for food?

Dear all. I have access to ankom fiber analyzer which is mostly useful for measuring ADF, NDF and crude fiber for animal food. I want to do preliminary test to see if my fruits have prebiotic...

01 February 2018 6,277 3 View

How much is the minimum volume of sample I need to take for SCFA analysis?

Hi everyone, I need to measure short chain fatty acids from my experiment, but my problem is limited volume of sample I have during the experiment. I know for HPLC of short chain fatty acid 20...

11 December 2017 8,389 2 View

Using bacteria suspension from freeze dried is the same as making suspension from back up colony?

Hi everyone, I received a Bifidobacterium lactis in freeze dried form, I revived it in media and I took its growth curv. Also, I did streak plating to have the colonies as back up and put in the...

11 December 2017 9,050 0 View

Prebiotic activity test ?

11 December 2017 2,239 1 View

How to sterilize fruit powder before adding to media for a microbiology test?

Hi everyone, I have a solution ( containing fruit powder, media and probiotics) I need to calculate the growth of particular probiotics in my solution, to see if the fruit has prebiotic activity....

10 November 2017 2,236 11 View

Measuring prebiotic activity of a fruit

07 August 2017 1,950 5 View

Why methanolic extract of a specific flower to use as antioxidant in frying burns?

I have made methanolic extract of a specific flower to use as antioxidant in frying, but the moment I added it to the hot oil, it burned, while many papers have used the extract in temperature of...

06 July 2017 8,839 4 View

In total flavonoid test (Alcl3 test )Can we run standard solution and samples separately?

06 July 2016 6,659 3 View

Can anyne please answer my confusion about FRAP assay?

Different literatures read spectrophotometer in different time, I am very confused how and when to read the numbers of spectrophotometer and how to zero it. Please let me know if you can clarify...

06 July 2016 6,134 4 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View