Can anyne please answer my confusion about FRAP assay?

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What is the pH to reach in measuring titratable acidity?

Dear all, For measuring the titratable acidity, I see titration of sample with 0.1 N NaOH to reach specific pH and reported by lactic acid. But the specifi pH I see is different in literiture,...

02 March 2018 1,419 4 View

Can I use ADF NDF crude fiber values for food?

Dear all. I have access to ankom fiber analyzer which is mostly useful for measuring ADF, NDF and crude fiber for animal food. I want to do preliminary test to see if my fruits have prebiotic...

01 February 2018 6,499 3 View

How much is the minimum volume of sample I need to take for SCFA analysis?

Hi everyone, I need to measure short chain fatty acids from my experiment, but my problem is limited volume of sample I have during the experiment. I know for HPLC of short chain fatty acid 20...

11 December 2017 8,541 2 View

Using bacteria suspension from freeze dried is the same as making suspension from back up colony?

Hi everyone, I received a Bifidobacterium lactis in freeze dried form, I revived it in media and I took its growth curv. Also, I did streak plating to have the colonies as back up and put in the...

11 December 2017 9,249 0 View

Prebiotic activity test ?

Dear Friends, I want to measure prebiotic activity of some samples (by comparing growth of 3 bacteria (2 prbiotics and one E.coli ) on some samples to see if the samples are encouraging for good...

11 December 2017 2,430 1 View

How to sterilize fruit powder before adding to media for a microbiology test?

Hi everyone, I have a solution ( containing fruit powder, media and probiotics) I need to calculate the growth of particular probiotics in my solution, to see if the fruit has prebiotic activity....

10 November 2017 2,401 11 View

Measuring prebiotic activity of a fruit

Hi everyone, I want to measure prebiotic activity of a fruit powder ( using fecal microbiota), I am wondering if I measure short chain fatty acids at the end of the test , how can I know that...

07 August 2017 2,263 5 View

Why methanolic extract of a specific flower to use as antioxidant in frying burns?

I have made methanolic extract of a specific flower to use as antioxidant in frying, but the moment I added it to the hot oil, it burned, while many papers have used the extract in temperature of...

06 July 2017 9,005 4 View

In total flavonoid test (Alcl3 test )Can we run standard solution and samples separately?

Hi, I did the total flavonoid test but the standard solution curve does not seem good enough (R square 77 percent, Can I run just the standard sololution again? Or do I have to do the whole test...

06 July 2016 6,805 3 View

Suggestion fo standard and valid DPPH assay?

Confused about choosing which protocol among many protocols for different assays and this time about DPPH assay! please suggest me any good and well known DPPH protocol. And in general if you know...

06 July 2016 8,935 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Can the limit of quantification (LOQ) of an analytical method fall outside its linear dynamic range, or must it always be within it?

Can an analytical method's limit of quantification (LOQ) be outside its linear dynamic range, or is it always required to be within it? Please provide a thorough explanation supported by verified...

29 July 2024 7,198 9 View

Where to start the Analytical model for Dry sliding wear of metal surface ?

I am doing research on wear mechanism of the additive manufactured metal parts under dry condition. Please share the reference to start the analytical modelling of the wear prediction.

29 July 2024 6,115 0 View

How can I use DFT-D3 correction ( Siesta version 5.0.1)?

Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...

27 July 2024 5,748 0 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Is there a method to measure the concentration of Cs, Sr, Co using spectrophotometer?

it's important to know that we are using the inactive elements of Cs, Sr, Co

25 July 2024 1,553 2 View

How to determine method detection limit in an analytical method?

I know the difference between instrumental LOD and method LOD but my query is - in case of any sample whose concentration is zero or not detected by the instrumental LOD, is it possible to get...

24 July 2024 6,592 5 View

Mechanochemistry: ball to powder mass or ball to powder volume?

I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...

23 July 2024 9,938 1 View