Previously, I had read a mechanism about conditionally-skip transcription from a cloned cassette in an expression vector. But i can't find that paper. It was containing a cis-regulatory element as I remember.
Any one knows that mechanism?
Thanks in advance
I guess your are referring to the loxP-flanked transcriptional termination (Lox-Stop-Lox; LSL) element. Here is just one Paper that may be a good starting point for you (Chu et al. BMC Biotechnology (2016) 16:4 DOI 10.1186/s12896-016-0234-4).
There are many plasmids with LSL elements at Addgene.org
Thanks Dr. Uwe Borgmeyer
This is exactly what I mean; the Cre/loxP system.
Thanks
Floxing an expression cassette makes it able to be stopped (4HT induced excision), but not "restarted". Of course, you can also flox out something that interrupts transcription. This can be combined with a second loxP site so that you can turn on, and then turn off, but that is about as far as you can go with multiple loxP sites being used more than once.
Alternatively, the inducible promoter systems (e.g. Tet-on/off) allows, with some leakage, the ability to induce or stop transcription.
https://blog.addgene.org/plasmids-101-inducible-promoters
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