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Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
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Hello, I am trying to clone 1334 bp insert into 6.1 kb vector using HiFi DNA assmebly/Gibson assembly kit. I am taking 100ng of vector and 1:5 molar ratio of vector: insert and 4 hrs of...
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I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...
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Hello, I am trying to achieve the CRISPR KNOCK IN of HDR 1.7 kb in HEK293T will one sgRNA will be efficient to get a knock in or else should I use two sgRNAs?
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Hello, I am trying to generate the CRISPR Knock In of about 1.7kb in HEK293T Cell line my HDR(linearized) is having 500bp Right Homology Arm left, 500bp Right Homology Arm and 1.8kb transgene how...
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