Is running several one-way ANOVAs justifiable when an (appropriate) two-ways ANOVA does not reach statistical significance?

Hi, We have been discussing this topic on my lab for a long time. Is there any justification for reporting statistically significant results of several one-way ANOVAs when a previous and more...

29 January 2020 8,458 2 View

Choosing compound' doses for rats when only mice-related bibliography is available?

Hi all, We are planning to systemically deliver a synthetic pharmacological compound in rats. When looking for specific dosage and administration protocols, we only found studies with mice....

05 May 2019 3,644 5 View

TCID50 for Non-forming and Non-CPE virus?

Is there a possibility to calculate TCID50 for non-plaque forming virus, the virus I'm trying to titer is also known to be non-cytopathic, so my questions is: Is there a method of titers this sort...

15 April 2019 8,266 2 View

No title

10 March 2019 7,613 4 View

With SOLID185 microplane damage model. How do I solve my error in Ansys??

In the last weeks I have worked the damage model of the element SOLID65, but it has not allowed any results, so I decided to work with the element SOLID185, with the recommendations of the...

07 March 2019 5,662 5 View

Can someone help me with Immunofluorescence Assay for CSFV quantification?

I'm testing a protocol for viral quantification by IF; it is being standarizing from the fixation step, as a fixative choosen are Methanol (10mins) and 4% Formaldehyde-10%Methanol (15mins) (Is...

26 February 2019 3,020 1 View

What kind of body measures can I take in wasps to contrast differences between individuals from different nests?

27 January 2019 7,520 11 View

I need to extract dna from environmental biofilms from contaminated sites that solution recommend me to clean my samples?

biology

03 April 2017 9,126 0 View

Somebody can recommend me some Books?

I'll become a student (MSc by research in organic chemistry). I would like to know some useful books expecially for the "work in the laboratory" like spectroscopy, how to purify some reagents...

02 March 2017 2,429 0 View

What is the best way to recover low amounts of DNA from an agarose gel band?

10 November 2015 9,428 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View