What's the best way? to remove the wax covering layer of hemipterans (such as citrus psyllids), for Scanning Electron Microscopy?
Chloroform can help. For example, such protocol:
https://www.pgia.ac.lk/files/Annual_congress/journel/v26/Journal-No%202/Papers/2%20-62.%20Mr.%20U.G.A.I.%20Sirisena%20OK_new.pdf
Immerse in Chloroform for 1 hr to dissolve the surface wax, finishing with a final rinse in 100% ethanol (from a newly opened bottle, to ensure no water was present).
Good luck!
Thanks Denis, for kind information. -Mubasher
Dear Mubasher
Any organic solvents ,i.e., acetone, would do the job of cleaning the wax ,covering layer of hemipterans. Then you can dry it by normal air
prior SEM examinations.
Dear Khaled J. Habib,
Thanks for kind suggestion.
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