How to convert HIV Copies/mL to IU/mL ?

I am developing HIV Viral Load Detection Assay, in that my Quantitative Standards are in Copies/mL, but I want to convert it to International Unit (IU)/mL as WHO recommendation to give results in...

08 September 2019 2,928 4 View

Problem in HIV RT PCR, every samples and water also coming positive at same time. Can anyone suggest what is the problem.?

I am using primer & Probe concentration 10 uM and using 0.5 uL in 25 uL reaction PCR setup. I have used so many different water but each water coming positive. Its might be primer dimer but...

01 February 2019 4,049 11 View

Which stabilizer we can used to stabilized RT PCR Master Mix?

I have developed RT PCR mastermix , now its required to add stabilizer in it? if yes which stabilizer we can use at which concentration.

11 December 2018 4,533 10 View

Which are the suitable gene target for DNA Extraction Internal control for RT PCR of DNA Virus?

We want Internal Control for DNA RT PCR, which should be act as a DNA extraction control.

05 June 2018 3,586 4 View

What is the best way to design DNA/RNA Extraction control for RT PCR testing?

I want to know the artificial gene or strain which can be use as a DNA/RNA extraction control, and will lead you to RT PCR testing for any viral DNA or RNA.

03 April 2018 5,148 4 View

I am not getting Perfect Sigmoidal curve in RT PCR. Can any one suggest the Possible reasons?

Please suggest possible modifications in experiments.

10 November 2017 221 6 View

Can any one know stabilizer for PCR Master Mix?

I want to stabilize the RT PCR Master Mix at Room Temperature . Please suggest if any one having same experience or some stabilizer i can try.

09 October 2017 4,404 3 View

What is the best way to detect MicroRNA , Sybr Green or Taqman?

10 November 2015 821 8 View

What blocking buffers can I use for a Lateral Flow Immunoassay?

What blocking buffers can I use for a Lateral Flow Immunoassay? (NCM Blocking, after spraying)

06 July 2015 6,638 2 View

Problem in R for generating Heatmap.

i am getting following error in R while generating heatmap. Error in .External.graphics(C_layout, num.rows, num.cols, mat, as.integer(num.figures), : invalid graphics state Please Guide me.

01 February 2015 7,641 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View