For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

Which important microorganism which destroy deemak (Termite) in agriculture soil or land eg safed musali cultivation or any rhizome crop?

we have problem in our field experiment of safed musali (Chlorophytum borivilianum ) cultivation. there is attack of termite in the soil which damage the roots in plant. we approached for organic...

02 September 2019 8,488 1 View

Can PCR master mix solution be prepared in advance and stored at -20C?

For doing PCR, instead of preparing the PCR mater mix solution each and every time, is it advisable to prepare it in a larger quantity and store it at -20C so that the PCR can be set up faster....

27 January 2019 7,112 19 View

How deep learning with IoT can be applied in agriculture?suggest any specific application of agriculture ?

Application of agriculture

01 January 2019 9,107 3 View

How can I plot grains as a point in an inverse pole figure map using TSL OIM software?

A figure like the given one.

29 August 2017 3,374 2 View

How o calculate impedance in hfss using polar plot

I am trying get results from the hfss after design information an antenna and wanted to find if my impedance is matched or not.at 50om .so how can I do that....thanks in advance

18 May 2017 948 5 View

Can any one suggest me alternate substrate for alpha amylase (PPA) assay?

20 March 2017 5,761 3 View

Tieing Eulerian material to Lagrangian body/surface?

Hello, I am trying to tie a Eulerian object to a Lagrangian body or surface in order to give the Eulerian material translational and angular velocities using ABAQUS/CAE. In the ABAQUS...

07 March 2017 7,230 6 View

How to do deconvolution of peaks of XPS (X ray Photoelectron spectra)?

There are many softwares available, but can I know how to analyze XPS using origin or qtiplot etc plotting software?

01 February 2017 2,486 3 View

What is the reason for peak splitting in XRD? Can reitveld refinement be done for the same?

18 November 2016 8,730 16 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View