Disha Varma

4 Questions 5 Answers 0 Followers

Questions related from Disha Varma

How to remove fluorescent debris effectively from my 40uM brain section?

I have been working with 40uM mouse brain sections mounted on triple coated chrome alum gelatin coverglass for multiplex imaging. By Round 2 I see these fluorescent specs/precipitate/debris all...

18 July 2022 9,214 0 View

Does any have a protocol for conjuagting Fc specific Fab fragment?

Hello, does anyone working with antibody fragments (Fab and Fc) conjugation? I am new to this field and any help would be great. I have been working on conjugating primary antibodies but have been...

18 August 2021 2,535 3 View

How to co-incubate primary and secondary at the same time on FFPE sections?

I am looking to seek suggestions on how to pre-complex primary and conventional secondary or primary and conjugated secondary to use for IF on FFPE sections. Any tips, protocols, suggestions or...

18 August 2021 4,307 7 View

Is anyone currently working with HL-1 cardiac muscle cell line?

Bought the HL-1 cells from EMD Millipore early this month. Thawed the vial into a T-25 flask. All was going great and they were beating fine, until this one day where the cells overgrew and kind...

30 October 2018 9,341 0 View