For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

Apart from using probiotics, following biosecurity and good management practices, is there any cure/medicine for White feces disease in Vannamei now?

the gut related diseases like White feaces, white muscle, white gut are doing much damage to shrimp by retarding growth and increasing FCR and eventually heavy losses. is there any medicine or...

07 October 2020 5,213 3 View

How deep learning with IoT can be applied in agriculture?suggest any specific application of agriculture ?

Application of agriculture

01 January 2019 9,107 3 View

Can any one suggest me alternate substrate for alpha amylase (PPA) assay?

20 March 2017 5,761 3 View

Can anybody suggest a good Knoevenagel condensation procedure for ketones with malonates?

Condensation between acetophone and diethyl malonate.

06 February 2017 148 3 View

What is the reason for peak splitting in XRD? Can reitveld refinement be done for the same?

18 November 2016 8,730 16 View

How to generate generic fatigue load cases for chassis rear suspension if i have road load data for different tracks which represent the entire life ?

Currently i have road load data containing wheel transducer forces FX,Fy,FZ,MX,MY,MZ and strain gauge data at critical stress concentration zones. i want to develop generic load cases at wheel...

28 September 2016 6,118 2 View

Can we convert our crop plants into "Mesembryanthemum crystallinum" ?

It's a facultative CAM plant. If this machinery is transfered to crop plants they can easily withstand salt and water stress.

10 April 2016 7,098 6 View

What I want to know is how to calculate the dielectric constant of Binary mixtures theoretically ?

03 February 2016 1,585 2 View

Is docosahexeanoic acid (DHA) and BSA conjugation required for invivo exogenus docosahexaenoic acid ip administration?

18 August 2015 978 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View