For lc50 study on fish against bacteria, the test performed referring to OD reading at 625nm. Is it acceptable method?

I would like to clarify regarding a method used to perform LC50 to infect fish by a particular bacteria strain. I prepared an overnight bacteria culture, collect the pellet from overnight culture...

05 December 2020 7,220 1 View

Apart from using probiotics, following biosecurity and good management practices, is there any cure/medicine for White feces disease in Vannamei now?

the gut related diseases like White feaces, white muscle, white gut are doing much damage to shrimp by retarding growth and increasing FCR and eventually heavy losses. is there any medicine or...

07 October 2020 5,213 3 View

How deep learning with IoT can be applied in agriculture?suggest any specific application of agriculture ?

Application of agriculture

01 January 2019 9,107 3 View

Can any one suggest me alternate substrate for alpha amylase (PPA) assay?

20 March 2017 5,761 3 View

Can anybody suggest a good Knoevenagel condensation procedure for ketones with malonates?

Condensation between acetophone and diethyl malonate.

06 February 2017 148 3 View

What is the reason for peak splitting in XRD? Can reitveld refinement be done for the same?

18 November 2016 8,730 16 View

How to generate generic fatigue load cases for chassis rear suspension if i have road load data for different tracks which represent the entire life ?

Currently i have road load data containing wheel transducer forces FX,Fy,FZ,MX,MY,MZ and strain gauge data at critical stress concentration zones. i want to develop generic load cases at wheel...

28 September 2016 6,118 2 View

Can we convert our crop plants into "Mesembryanthemum crystallinum" ?

It's a facultative CAM plant. If this machinery is transfered to crop plants they can easily withstand salt and water stress.

10 April 2016 7,098 6 View

What I want to know is how to calculate the dielectric constant of Binary mixtures theoretically ?

03 February 2016 1,585 2 View

Is docosahexeanoic acid (DHA) and BSA conjugation required for invivo exogenus docosahexaenoic acid ip administration?

18 August 2015 978 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

What should be the duration of exposure to simulated space environments with Rodents?

Hi, I am currently working on a pilot study to get some data related to the impact of long duration spaceflight to the microbiome and the immune system. I plan to use rats and simulate...

22 February 2021 592 1 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View