My PCR samples (50μl) were cleaned by spin column, and eluted with equal volumes of Tris buffer (50μl) (~8 ng/μl).
The eluent contains target fragment (~500 bp) and primer dimers (~100 bp). [Figures attached]
How can I remove primer dimers (~100 bp) with ethanol preciptation method ?
Or other method suggested to remove primer dimers (~100 bp DNA fragment).
[Figure legend]
#numbers are desinated for sample numbers.
"N" for negative control (PCR without template DNA).
I would suggest to do in gel digestion of your bands. If you are afraid of getting low yield then elute it in 6 to 10 ul- Even after this it is less then after in gel digestion you can amplify amplicon.
Hi Frank,
have a look to this comprehensive lecture about your question:
Conference Paper Primer-Dimer Formation: �The Problem and the Solution
Hope it helps.
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