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Questions related from Frank Zhuang
My PCR samples (50μl) were cleaned by spin column, and eluted with equal volumes of Tris buffer (50μl) (~8 ng/μl). The eluent contains target fragment (~500 bp) and primer dimers (~100 bp)....
29 November 2018 6,225 2 View
In my protocol, balb/c female mices were divided into two groups : 1. PBS group 2. OVA group which were immunized with PBS or OVA antigen respectively by introperitoneal injection on day 15, 25,...
13 September 2018 1,918 0 View
I've been recently using GE Sepharose® CL-6B gel (an size-exclusion gel) to separate polysaccharide samples. But I accidentally pumped air into the column and dried out the well-packed gel inside...
20 August 2018 9,554 6 View
