What do you need it for?

First option is to use chemically-breakable PAGE. There are cross linkers, which can be broken with some reagents.

Second, which I used, is simply to press the gel e.g. through syringe several times and then extract the protein into desired buffer/solution.

Since the protein is already denatured, it doesn't matter that much what buffer you use.

actually i have inactivated rabies virus i.e- whole virus but i want glycoprotein bcoz its only the antigenic part of virus.So i want only that part.

I see. Then use any of the two options. The second one probably won't give you 100% yield but it depends, whether you want to spend money for the extra chemicals :)

Ok then i will try it. Thanks