I want to excise glycoprotein from SDS-PAGE which is 66 KD. Does anybody have a protocol that describes how to excise it from SDS-PAGE and finally in which buffer I should store it?
What do you need it for?
First option is to use chemically-breakable PAGE. There are cross linkers, which can be broken with some reagents.
Second, which I used, is simply to press the gel e.g. through syringe several times and then extract the protein into desired buffer/solution.
Since the protein is already denatured, it doesn't matter that much what buffer you use.
actually i have inactivated rabies virus i.e- whole virus but i want glycoprotein bcoz its only the antigenic part of virus.So i want only that part.
I see. Then use any of the two options. The second one probably won't give you 100% yield but it depends, whether you want to spend money for the extra chemicals :)
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