How to separate rabies virus glycoprotein?

I want to separate rabies virus glycoprotein. Can anybody suggest purification method and protocol?

31 December 2012 482 4 View

How to purifye glycoprotein from SDS-PAGE? Which buffer used for its final storage?

I want to excise glycoprotein from SDS-PAGE which is 66 KD. Does anybody have a protocol that describes how to excise it from SDS-PAGE and finally in which buffer I should store it?

04 May 2012 7,661 4 View

I am working on gas gangrine toxin production by static condition and i am facing problem with its activity?

I am working on toxin production responsible for gas gangrine by using clostridium perfringens in static flask condition but i will able to get only small amount of toxin by this method. Can any...

11 December 2011 3,203 2 View

How to check phospholipase c activity with zymogram?

As i tried with zymogram by incorporation of lecithin in running gel and Silver staining but i didnot find it. so please provide me accurate protocol for this.

10 November 2011 8,079 0 View

how caprylic acid precipitate non specific protein except Immunoglobulin G (Ig G) from plasma? what is mechanism behind that?

during antisera production first plasma sent for pepsin digestion and then for caprylic acid precipitation during which caprylic acid precipitate only non specific protein except IgG.

08 September 2011 3,697 4 View

Is it possible to grwo anaerobic bacteria like clostridium perfringens without use of higly desined anerobic chamber?

Actually we had anaerobic chamber but because of some disturbance it will not able to maintain anaerobic condition inside the chember i.,e it is leaking and we contacted our service engineer but...

07 August 2011 6,571 4 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View