As per above question i am interested in PEG and phosphate recipe for ATPS of toxin , can any one suggest me how to proceed for that ?
I want to separate rabies virus glycoprotein. Can anybody suggest purification method and protocol?
31 December 2012 549 4 View
I want to excise glycoprotein from SDS-PAGE which is 66 KD. Does anybody have a protocol that describes how to excise it from SDS-PAGE and finally in which buffer I should store it?
04 May 2012 7,729 4 View
I am working on toxin production responsible for gas gangrine by using clostridium perfringens in static flask condition but i will able to get only small amount of toxin by this method. Can any...
11 December 2011 3,273 2 View
As i tried with zymogram by incorporation of lecithin in running gel and Silver staining but i didnot find it. so please provide me accurate protocol for this.
10 November 2011 8,143 0 View
during antisera production first plasma sent for pepsin digestion and then for caprylic acid precipitation during which caprylic acid precipitate only non specific protein except IgG.
08 September 2011 3,811 4 View
Actually we had anaerobic chamber but because of some disturbance it will not able to maintain anaerobic condition inside the chember i.,e it is leaking and we contacted our service engineer but...
07 August 2011 6,696 4 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
I was wondering if it is possible to form a permanent open "ssDNA bubble" similar to a transcription bubble (>13 nucleotides) within E. coli. These criteria are important: 1. Open ssDNA...
07 July 2024 8,275 5 View
Give in detail with available literature and website links for submissions
27 June 2024 9,753 1 View
Hey everyone! I'm having difficulty marking the C4 complement factor in Western Blotting. Has anyone had contact with this antibody or made it in-house? I have already used Anti-C4β (D-12)...
17 June 2024 6,124 1 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I was curious as to why modern soybean half-seed protocols use 1/10x strength B5 salts during the co-cultivation step rather than full strength B5 salts? Also, are the B5 vitamins added at full...
07 May 2024 3,924 0 View
I am following the standard method which is mentioned in Tomotake Kanki et al., 2009 research paper Monitoring mitophagy in yeast: The Om45-GFP processing assay . I am growing the cells in SD-HIS...
04 May 2024 6,463 0 View
How are these exploited in biotechnological applications?
07 April 2024 566 5 View