As i tried with zymogram by incorporation of lecithin in running gel and Silver staining but i didnot find it. so please provide me accurate protocol for this.
I want to separate rabies virus glycoprotein. Can anybody suggest purification method and protocol?
31 December 2012 482 4 View
I want to excise glycoprotein from SDS-PAGE which is 66 KD. Does anybody have a protocol that describes how to excise it from SDS-PAGE and finally in which buffer I should store it?
04 May 2012 7,661 4 View
As per above question i am interested in PEG and phosphate recipe for ATPS of toxin , can any one suggest me how to proceed for that ?
01 February 2012 1,281 0 View
I am working on toxin production responsible for gas gangrine by using clostridium perfringens in static flask condition but i will able to get only small amount of toxin by this method. Can any...
11 December 2011 3,203 2 View
during antisera production first plasma sent for pepsin digestion and then for caprylic acid precipitation during which caprylic acid precipitate only non specific protein except IgG.
08 September 2011 3,697 4 View
Actually we had anaerobic chamber but because of some disturbance it will not able to maintain anaerobic condition inside the chember i.,e it is leaking and we contacted our service engineer but...
07 August 2011 6,571 4 View
If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...
26 February 2021 3,526 3 View
I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .
22 February 2021 1,131 4 View
Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...
20 February 2021 6,897 1 View
I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...
15 February 2021 7,444 2 View
I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...
14 February 2021 7,800 7 View
I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...
09 February 2021 8,594 3 View
In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...
08 February 2021 2,792 1 View
Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...
07 February 2021 9,530 3 View
I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...
04 February 2021 1,509 3 View
Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...
04 February 2021 8,993 2 View