How to separate rabies virus glycoprotein?

I want to separate rabies virus glycoprotein. Can anybody suggest purification method and protocol?

31 December 2012 482 4 View

How to purifye glycoprotein from SDS-PAGE? Which buffer used for its final storage?

I want to excise glycoprotein from SDS-PAGE which is 66 KD. Does anybody have a protocol that describes how to excise it from SDS-PAGE and finally in which buffer I should store it?

04 May 2012 7,661 4 View

Can any one advise me how to proceed for toxin purification from clostridium perfringens fermented broth by aqueous two phase system?

As per above question i am interested in PEG and phosphate recipe for ATPS of toxin , can any one suggest me how to proceed for that ?

01 February 2012 1,281 0 View

I am working on gas gangrine toxin production by static condition and i am facing problem with its activity?

I am working on toxin production responsible for gas gangrine by using clostridium perfringens in static flask condition but i will able to get only small amount of toxin by this method. Can any...

11 December 2011 3,203 2 View

How to check phospholipase c activity with zymogram?

As i tried with zymogram by incorporation of lecithin in running gel and Silver staining but i didnot find it. so please provide me accurate protocol for this.

10 November 2011 8,079 0 View

Is it possible to grwo anaerobic bacteria like clostridium perfringens without use of higly desined anerobic chamber?

Actually we had anaerobic chamber but because of some disturbance it will not able to maintain anaerobic condition inside the chember i.,e it is leaking and we contacted our service engineer but...

07 August 2011 6,571 4 View

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences?

Greetings to all, has any body published a scientific paper in EuroAsian Journal of BioSciences and if so could you please tell me if there was any problems regarding communications with the...

05 October 2020 1,713 2 View

Do you agree with the just issued Great Barrington Declaration regarding the response to COVID-19?

The short and to-the-point text is here and I for one gladly co-signed this declaration, just as I also co-signed the balanced approach statement issued in July 2020 by 23 public health experts...

04 October 2020 8,795 11 View

The meaning of protein phosphorylation to bioscience?

Many researchers have mentioned in their researches that protein phosphorylation take a decisive position to regulate various physiological activity, from prokaryote to eukaryote. So did...

23 July 2020 7,135 3 View

Is there any cause of pellet from the culturing media?

11 June 2020 3,585 3 View

How do you reduce the static forces in canon ball packing of fruits for transportation?

Examples of how to increase the contact surface area of the bottom area? Thank you.

25 March 2020 9,187 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

03 January 2020 3,565 4 View

How molecular genetics could be useful for farmers?

Molecular genetics is the branch of genetics that investigates the chemical and physical nature of genes and the mechanisms by which genes control development, growth and physiology. It is the...

31 December 2019 3,792 4 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

22 September 2019 2,889 3 View

Has anyone successfully stained (intracellular staining) for T-bet with the Cytofix/Cytoperm Kit from BD Biosciences??

I isolate PBMCs from human blood and try to perform intracellular staining for T-bet (i'm interested in CD19+ cells, but for now i use CD3+ as a control). I do know there are kits that are...

08 September 2019 5,009 3 View

So what you think that either you will choose to develop a desktop application, mobile application or the application that can run on both platforms ?

As internet usage increase web application development become a challenge, because it is mostly customer requirement that web application must be usable on both desktop computer and...

07 August 2019 1,193 3 View