Avinash Gaikwad

7 Questions 7 Answers 0 Followers

Questions related from Avinash Gaikwad

How to separate rabies virus glycoprotein?

I want to separate rabies virus glycoprotein. Can anybody suggest purification method and protocol?

01 January 2013 521 4 View

How to purifye glycoprotein from SDS-PAGE? Which buffer used for its final storage?

I want to excise glycoprotein from SDS-PAGE which is 66 KD. Does anybody have a protocol that describes how to excise it from SDS-PAGE and finally in which buffer I should store it?

05 May 2012 7,704 4 View

Can any one advise me how to proceed for toxin purification from clostridium perfringens fermented broth by aqueous two phase system?

As per above question i am interested in PEG and phosphate recipe for ATPS of toxin , can any one suggest me how to proceed for that ?

02 February 2012 1,314 0 View

I am working on gas gangrine toxin production by static condition and i am facing problem with its activity?

I am working on toxin production responsible for gas gangrine by using clostridium perfringens in static flask condition but i will able to get only small amount of toxin by this method. Can any...

12 December 2011 3,239 2 View

How to check phospholipase c activity with zymogram?

As i tried with zymogram by incorporation of lecithin in running gel and Silver staining but i didnot find it. so please provide me accurate protocol for this.

11 November 2011 8,114 0 View

how caprylic acid precipitate non specific protein except Immunoglobulin G (Ig G) from plasma? what is mechanism behind that?

during antisera production first plasma sent for pepsin digestion and then for caprylic acid precipitation during which caprylic acid precipitate only non specific protein except IgG.

09 September 2011 3,759 4 View

Is it possible to grwo anaerobic bacteria like clostridium perfringens without use of higly desined anerobic chamber?

Actually we had anaerobic chamber but because of some disturbance it will not able to maintain anaerobic condition inside the chember i.,e it is leaking and we contacted our service engineer but...

08 August 2011 6,648 4 View