As the title, How could we know the protein of interest binds with DNA or RNA?
Is there any binding assay could be used?
Here, I am using HEK293T cells to product lentivirus. The plasmid system is pCDH, pMG-VSVG, pRSV-Rev and pMDLg-PRRL. I can get 1x 10^8 viruses from 10-cm dish HEK293T cells. It is not enough for...
08 September 2017 3,930 0 View
Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ? Here, I used LentiCRISPR-v2 system to harvest virus carring guide RNA, but when I added the virus into THP1...
10 November 2016 6,351 2 View
We always use HEK293A cells to package adenvirus, could HEK293T cells be used to do it? Thank you~~~
08 September 2016 8,266 7 View
Read 2 answers by scientists with 3 recommendations from their colleagues to the question asked by Yongxu Zhao on Sep 25, 2016
08 September 2016 9,204 2 View
As title, is there any great protocol to do this experiment?
01 February 2016 3,552 0 View
THP1 is the famous monocyte cell line, however which is hard to be transfected. Are there any other cell lines which is similar with THP1 and easier to be transfected?
01 February 2016 6,220 5 View
When we construct plasmids, empty vectors containing RFP and dsRed were used usually, so what is the difference between each other?
04 May 2015 8,054 6 View
We know beta-catenin binds with E-Cadherin intracellular domain stably, while Wnt pathway is activated, beta-catenin will be released from membrane, however, what is the mechanism?
09 October 2014 4,171 4 View
Both the structure and sequence of E-Cadherin are similar with those of N-Cadherin. However the function of these two cadherins is totally different. Why?
06 July 2014 209 9 View
Does anyone have a detailed protocol for macrophage and cancer cell cocultures?
10 November 2013 9,262 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View