14 Questions 14 Answers 0 Followers
Questions related from Yongxu Zhao
Here, I am using HEK293T cells to product lentivirus. The plasmid system is pCDH, pMG-VSVG, pRSV-Rev and pMDLg-PRRL. I can get 1x 10^8 viruses from 10-cm dish HEK293T cells. It is not enough for...
09 September 2017 3,906 0 View
Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ? Here, I used LentiCRISPR-v2 system to harvest virus carring guide RNA, but when I added the virus into THP1...
11 November 2016 6,324 2 View
We always use HEK293A cells to package adenvirus, could HEK293T cells be used to do it? Thank you~~~
09 September 2016 8,246 7 View
Read 2 answers by scientists with 3 recommendations from their colleagues to the question asked by Yongxu Zhao on Sep 25, 2016
09 September 2016 9,183 2 View
As the title, How could we know the protein of interest binds with DNA or RNA? Is there any binding assay could be used?
08 August 2016 3,226 0 View
THP1 is the famous monocyte cell line, however which is hard to be transfected. Are there any other cell lines which is similar with THP1 and easier to be transfected?
02 February 2016 6,199 5 View
As title, is there any great protocol to do this experiment?
02 February 2016 3,524 0 View
When we construct plasmids, empty vectors containing RFP and dsRed were used usually, so what is the difference between each other?
05 May 2015 8,034 6 View
We know beta-catenin binds with E-Cadherin intracellular domain stably, while Wnt pathway is activated, beta-catenin will be released from membrane, however, what is the mechanism?
10 October 2014 4,150 4 View
Both the structure and sequence of E-Cadherin are similar with those of N-Cadherin. However the function of these two cadherins is totally different. Why?
07 July 2014 189 9 View
Does anyone have a detailed protocol for macrophage and cancer cell cocultures?
11 November 2013 9,238 1 View
I cultured my THP1 cell using a conditional medium without FBS after PMA activation, and precipitated a protein by Chloroform Methanol Precipitation. When the membrane is incubated with...
08 August 2013 2,640 3 View
I want to do one protein secretion detected by Western blotting, so I harvested supernatant protein, but I don't know which protein to use as a control. We all use actin or tubulin as a control in...
08 August 2013 918 5 View
Mice Serum was harvest and run SDS-PAGE gel, membrane was incubated by anti-tubulin antibody (sigma), Why are there tubulin bands? Is tubulin secreted into serum?
08 August 2013 4,071 4 View
