As title, is there any great protocol to do this experiment?
Here, I am using HEK293T cells to product lentivirus. The plasmid system is pCDH, pMG-VSVG, pRSV-Rev and pMDLg-PRRL. I can get 1x 10^8 viruses from 10-cm dish HEK293T cells. It is not enough for...
08 September 2017 3,930 0 View
Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ? Here, I used LentiCRISPR-v2 system to harvest virus carring guide RNA, but when I added the virus into THP1...
10 November 2016 6,351 2 View
We always use HEK293A cells to package adenvirus, could HEK293T cells be used to do it? Thank you~~~
08 September 2016 8,266 7 View
Read 2 answers by scientists with 3 recommendations from their colleagues to the question asked by Yongxu Zhao on Sep 25, 2016
08 September 2016 9,204 2 View
As the title, How could we know the protein of interest binds with DNA or RNA? Is there any binding assay could be used?
07 August 2016 3,284 0 View
THP1 is the famous monocyte cell line, however which is hard to be transfected. Are there any other cell lines which is similar with THP1 and easier to be transfected?
01 February 2016 6,220 5 View
When we construct plasmids, empty vectors containing RFP and dsRed were used usually, so what is the difference between each other?
04 May 2015 8,054 6 View
We know beta-catenin binds with E-Cadherin intracellular domain stably, while Wnt pathway is activated, beta-catenin will be released from membrane, however, what is the mechanism?
09 October 2014 4,171 4 View
Both the structure and sequence of E-Cadherin are similar with those of N-Cadherin. However the function of these two cadherins is totally different. Why?
06 July 2014 209 9 View
Does anyone have a detailed protocol for macrophage and cancer cell cocultures?
10 November 2013 9,262 1 View
What are the emerging trends and opportunities for STEM education and careers in the online space?
10 August 2024 507 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
