How is beta-catenin released from E-Cadherin?

More Yongxu Zhao's questions See All

How to improve the production efficiency of lentivirus?

Here, I am using HEK293T cells to product lentivirus. The plasmid system is pCDH, pMG-VSVG, pRSV-Rev and pMDLg-PRRL. I can get 1x 10^8 viruses from 10-cm dish HEK293T cells. It is not enough for...

08 September 2017 3,930 0 View

Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ?

Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ? Here, I used LentiCRISPR-v2 system to harvest virus carring guide RNA, but when I added the virus into THP1...

10 November 2016 6,351 2 View

Could HEK293T cells be used to package adenovirus?

We always use HEK293A cells to package adenvirus, could HEK293T cells be used to do it? Thank you~~~

08 September 2016 8,266 7 View

What is the function of SV40 poly(A) sequence in one plasmid? Does it regulate the gene of interest that is cloned into the plasmid?

Read 2 answers by scientists with 3 recommendations from their colleagues to the question asked by Yongxu Zhao on Sep 25, 2016

08 September 2016 9,204 2 View

How to prove one protein binding with DNA or RNA?

As the title, How could we know the protein of interest binds with DNA or RNA? Is there any binding assay could be used?

07 August 2016 3,284 0 View

In THP1 cells, how is a gene knocked out using CRISPR/Cas9?

As title, is there any great protocol to do this experiment?

01 February 2016 3,552 0 View

Which cells are similar with THP1 and are easier to be transfected?

THP1 is the famous monocyte cell line, however which is hard to be transfected. Are there any other cell lines which is similar with THP1 and easier to be transfected?

01 February 2016 6,220 5 View

What is the difference between RFP and dsRed?

When we construct plasmids, empty vectors containing RFP and dsRed were used usually, so what is the difference between each other?

04 May 2015 8,054 6 View

Why is the function of E-Cadherin totally different from N-Cadherin?

Both the structure and sequence of E-Cadherin are similar with those of N-Cadherin. However the function of these two cadherins is totally different. Why?

06 July 2014 209 9 View

How to coculture macrophage and cancer cell?

Does anyone have a detailed protocol for macrophage and cancer cell cocultures?

10 November 2013 9,262 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

Goodwin G. Jinesh

Caspase-3 cleavage of beta-Catenin

Yongxu Zhao

Hi, Goodwin. is beta-catenin cleaved by capase-3, or E-Cadherin intracellular domain. also is caspase 3 regulated by Wnt signaling?

Here is the paper describing answer for your question

http://jcb.rupress.org/content/139/3/759.full.pdf+html

thank you very much~~~

But I also want to know whether Wnt could increase caspase3 activity?