11 November 2013 1 9K Report

Does anyone have a detailed protocol for macrophage and cancer cell cocultures?

More Yongxu Zhao's questions See All
How to improve the production efficiency of lentivirus?

Here, I am using HEK293T cells to product lentivirus. The plasmid system is pCDH, pMG-VSVG, pRSV-Rev and pMDLg-PRRL. I can get 1x 10^8 viruses from 10-cm dish HEK293T cells. It is not enough for...

08 September 2017 3,930 0 View

Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ?

Is there any protocol of knocking out one gene in THP1 cells using CRISPR/Cas9 system ? Here, I used LentiCRISPR-v2 system to harvest virus carring guide RNA, but when I added the virus into THP1...

10 November 2016 6,351 2 View

Could HEK293T cells be used to package adenovirus?

We always use HEK293A cells to package adenvirus, could HEK293T cells be used to do it? Thank you~~~

08 September 2016 8,266 7 View

What is the function of SV40 poly(A) sequence in one plasmid? Does it regulate the gene of interest that is cloned into the plasmid?

Read 2 answers by scientists with 3 recommendations from their colleagues to the question asked by Yongxu Zhao on Sep 25, 2016

08 September 2016 9,204 2 View

How to prove one protein binding with DNA or RNA?

As the title, How could we know the protein of interest binds with DNA or RNA?  Is there any binding assay could be used?

07 August 2016 3,284 0 View

In THP1 cells, how is a gene knocked out using CRISPR/Cas9?

As title, is there any great protocol to do this experiment?

01 February 2016 3,552 0 View

Which cells are similar with THP1 and are easier to be transfected?

THP1 is the famous monocyte cell line, however which is hard to be transfected. Are there any other cell lines which is similar with THP1 and easier to be transfected?

01 February 2016 6,220 5 View

What is the difference between RFP and dsRed?

When we construct plasmids, empty vectors containing RFP and dsRed were used usually, so what is the difference between each other?

04 May 2015 8,054 6 View

How is beta-catenin released from E-Cadherin?

We know beta-catenin binds with E-Cadherin intracellular domain stably, while Wnt pathway is activated, beta-catenin will be released from membrane, however, what is the mechanism? 

09 October 2014 4,171 4 View

Why is the function of E-Cadherin totally different from N-Cadherin?

Both the structure and sequence of E-Cadherin are similar with those of N-Cadherin. However the function of these two cadherins is totally different. Why?

06 July 2014 209 9 View

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