How to produce high titer retrovirus with pCL-ECO and pMIG packaging system in 293t cell line?could you give me some advice?

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Rakesh Kumar

Again reinfect the cell extract in different 293

Nicolas Grandchamp

Hi Peng,

In my experience the protocol based on ultra-centrifugation are the best to obtain the most efficient lentiviral vectors.

Another way is to contact companies which have a strong know-how to produce ultra high efficientvectors ;)

Regards

Nicolas