During annealing of spin-coated ITO, is annealing in vacuum better than annealing in air?

I am considering mainly about conductivity and trasmittance. If annealing in vacuum is better, why is it?

17 November 2019 1,835 3 View

DNA agarose gels without a dark room?

Hi. Unfortunately our lab doesn't have a dark room, so when we are cutting bands from our Sybr-Safe or Midori Green stained gels, we often find it difficult to see the bands present which are...

06 June 2019 8,230 1 View

MRNA 3' sequence after in vitro transcription of linear DNA template?

Hi. I'm looking in to doing somein vitro transcription from a linear dsDNA template, or a linearised plasmid. When my T7 RNA pol runs along generating the mRNA, once its hits the 3' end of the...

30 May 2019 9,010 3 View

Can I use penicillin/streptomycin in a hygromycin selection process?

Hi all, I'm working on preparation of a stable cell line using hygromycin selection process. I'm using HEK293 cell line and 200 ug /mL of hygromycin-containing media. However, I found that all...

25 September 2018 2,693 3 View

Can i ues simethicone with surfactant?

Dear researchers. I have to coat tablets with simethicone oil. Since simethicone is too oily coating quality is very poor. So, i thinking of adding surfactant. (SLS or tween) The question...

16 September 2018 5,636 2 View

How to produce high titer retrovirus with pCL-ECO and pMIG packaging system in 293t cell line?could you give me some advice?

could you kindly provide a detailed procedure?

22 April 2018 7,952 2 View

How vital is it to optimise total RNA Input for qPCR reactions?

How vital is it to optimize the RNA input for reverse transcription for subsequent qPCR reactions? The qPCR reaction mix protocol calls for 25ng of template. I've been using 100ng total RNA for...

05 March 2018 1,622 4 View

How can you fix cells without removing media?

I grow my neurons on glass surface. Their axons so delicate that I cannot remove the media entirely. When I refeed them, I remove half the media and replace it with fresh media containing double...

03 February 2018 1,918 3 View

Can I freeze a CoPi/ metal oxide in N2 before cutting it cross section for SEM?

Planning to do it this way, but cannot help worrying about possible changes that will happen to Co-Pi coating.

08 January 2018 5,440 2 View

Can I produce a significant amount of gypsum if I add sulfuric acid even before slaking of CaO occurs?

The paper attached claims to have produced around 600 g gypsum from 1 kg of egg shells. They did this by simultaneously adding CaO derived from calcination, water, and the sulfuric acid solution....

11 December 2017 7,452 1 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View

Problem in detection of a specific gene & elimination of primer dimer in qpcr?

In our lab, we are trying to do real time PCR for some genes. One of them has a size of 85 bp. Whatever we do, we had an amplification in negative control. When we run agarose gel, it always a...

14 February 2021 9,750 11 View

How to increase bacterial concentration of DNA from Laser capture microdissection samples?

I used laser capture microdissection, selected specific bacteria presented in ethanol-fixed slides. And DNA was extracted by Qiamp mini kit. The problem right now is we have pretty low...

09 February 2021 3,908 2 View

Why non-specific band appear on T-ARMS PCR?

Hi everyone. We want to know how can I remove the non-specific band amplification on this variant. Although the amplification of non-specific band is not as intense as the band that we want, but...

09 February 2021 588 2 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Xrd result of ZnO thin film shown better crystallinity at low temperature and the crystallinity decreases as the temperature increased. why?

I have synthesized ZnO thin films at four different substrate temperature say, 300, 350, 400 and 450C. and the samples were annealed at 450C for half an hour. the xrd result shown better...

04 February 2021 7,641 4 View

How to measure cosine, abbe, deadpath errors?

Dear sir, How to measure cosine, abbe, deadpath errors. I know cosine error, abbe error, deadpath error occurs in laser measurement. But how i can identify the cosine error value. Is any separate...

03 February 2021 4,878 4 View

How to apply graphene oxide on an gold electrode?

Dear Fellows, now it is the time to push graphene applications further in terms of easy and low cost production surroundings. Thus I recently dived in the materia and I am facing tons of papers,...

02 February 2021 6,284 2 View

How can I partially remove the metal oxide layer for solar cell device?

Hi, I want to make a perovskite-based planar solar cell device. (electrode/HTL/perovskite/ETL/FTO) To fabricate the device, one electrode should be deposited on HTL and the other should be...

01 February 2021 229 6 View