Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

I need dataset for recommandation system?

Hi every body I need to collect datasets from different source to work on recommendation system algorithms, could you advise me how collect data?

27 February 2021 7,063 7 View

How to estimate 2SLS with different instruments for different endogenous variables?

To be specific, I want to conduct an IV regression in stata with the following three equations. EQ1: Y1 ~Y2 Y3 X EQ2: Y2 ~ Z1 EQ3: Y3 ~ Z2 Both Y2 and Y3 are endogenous. However, Z1 and Z2 are...

23 February 2021 6,266 3 View

Which microtomy technique do you suggest for thin and delicate root sectioning ?And, how to preserve and process the roots before sectioning?

We have thin roots of from 1 year old different species of plants. We are looking into anatomical details of the roots. What's the best method to preserve and process the roots once they are...

23 February 2021 1,898 1 View

How to find out the problem of VMD software when i run it?

Hello I try to install and run the VMD software on windows 10 (64-bit).It doesn`t work and it is closed when I run it. I checked all of tutorials, videos and documents. But they did not work...

22 February 2021 8,211 3 View

Sample modeling in ABAQUS software and its relationship with the real world?

About crack modeling in ABAQUS: Mode I (pure-tensile cracking) and Mode II (pure-shear cracking), these assumptions may be accruing in theoretical approach (ABAQUS) not practical in Lab....

19 February 2021 6,156 1 View

Making my research papers available for readers?

17 February 2021 8,533 2 View

How does a freeze dryer destruct your solid sample?

Several times I lost my sample during freeze drying. There was no solid or powder in the vial after freeze drying. I'm sure sample contained solute because i took its NMR spectrum. I covered the...

15 February 2021 6,749 2 View

How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

How the precipitation Ratio and flow Ratio effect the output of the result?

I am doing HEC HMS calibration than i want to increase simulated value and volume of the computed so that my issue is how the precipitation and flow ratio make influential that result?

22 January 2021 152 2 View

Can anyone please tell me, what will be the Crop Coefficient value for shortleaf pine and oak tree in HEC-HMS model simulation?

my study area located in the forest region having Shortleaf Pine and Oaktree, thus in the Canopy Method I am trying to get the Crop Coefficient value of these trees but did not get anything.

18 January 2021 9,789 1 View

HEC RAS: project file error?

When I try to create a new project file on HEC RAS, this message appears "title and file name must be specified", even when I have already specified them.

03 January 2021 5,618 2 View

How to download future precipitation data for years 2021-2030?

For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?

21 December 2020 8,928 5 View

How to perform snowmelt runoff modeling in hEC HMS?

My study area of rainfall-runoff modelling is in mountainous region and has snow cover. There is about 10 percent snowmelt contribution to the discharge of the river i am modelling. How can I...

23 November 2020 7,873 4 View

How to solve the longest flow path error in HEC-geo HMS?

I'm having issues running the Longest Flow Path tool in HEC geo HMS. Attempting to run the Longest Flow Path tool results in the following warning error message. The error is attached. All...

19 August 2020 9,365 4 View

How can i classify these soils obtained from SOTER database to different Hydrological soil groups reguired in HEC HMS CN method??

I dont have any other soil maps for my study area.. How can i use this data to convert it into Hydrological soil groups A,B,C or D ?

04 August 2020 7,848 6 View

How do I determine the correct ratio to peak value for the Recession baseflow in HEC-HMS?

I am wondering if there is a way to estimate the ratio to peak value without calibration? What would be the most suitable value if I don't have a measured hydrograph to compare? Thanks for any...

27 July 2020 2,242 1 View

How do I estimate initial baseflow for each subbasin (HEC-HMS) ?

I have one hydrograph close to the watershed outlet from which I can extract a baseflow value. But what values would I assign to all the other subbasins? The HEC-HMS user manual says the...

21 July 2020 5,431 3 View