I tried with DI water but it cannot fully dissolve melatonin.Some people suggested to use little ethanol. But i have a confusion that if i use ethanol then it may have interference duringelectrochemical detection.I need some good suggestions.
Dear Shamim Ahmed Hira, please find attached an information sheet detailing the solubility properties of melatonin. Apparently pure water is not a good choice.
According to the specification sheet given by Santa cruz biotechnology it's solubility in water (0.1 mg/ml), ethanol (8 mg/ml), benzene, chloroform, methanol, DMSO (50 mM)