The goal of the experiment is to analyze cell cycle after adding a drug.nthanks
In order to provide statistical analysis, you should run in triplicate, the negative and positive control, and the cells treated. If you are going to use a kit for cell cycle analysis, following the manufacturer's instructions you should have no problems. If you are going to stain only with PI, usually 1.105 cells are accepted.
In order to provide statistical analysis, you should run in triplicate, the negative and positive control, and the cells treated. If you are going to use a kit for cell cycle analysis, following the manufacturer's instructions you should have no problems. If you are going to stain only with PI, usually 1.105 cells are accepted.
I agree with Ana and would like to add that you need to collect enough events to accurately measure each sub-population. Try at least 10e4 events. Look at the S and G2 populations to make sure they are well represented.
i am using six well plate so i am using duplicate not replicate. would this work? two wells for treated and two positive control and two wells negative control.
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line