How long prepared PBS 7.0 (Phosphate Buffered Saline) can be stored?

More Muhammad Saleem Kalhoro's questions See All

Can we judge the quality of scientific journal by its impact factor alone?

What are other good indicators of quality journal apart from high impact factor

10 November 2018 7,971 24 View

How to prepare bacterial culture for Scanning Electron Microscope (SEM) study?

I want to prepare lactic acid bacteria from SEM study

31 December 2017 2,320 3 View

How to save Excel graph as image with 300 DPI?

I tried both MS paint and MS word, then saved as image but DPI is 96, conversion from 96 to 300 DPI through software dose not improve image quality, I am looking for direct method to save Excel...

08 September 2017 1,900 43 View

How to prepare 20 mM sodium acetate buffer (pH 4.6)?

How to prepare 20 mM sodium acetate buffer (pH 4.6)

06 July 2017 8,425 4 View

How to prepare 100 ml HCL-KCL 10 mM buffer?

How to prepare 100 ml HCL-KCL 10 mM buffer, pH 4.6Will there be a change in pH after autoclave?

04 May 2017 9,292 2 View

How to add Tertiary Vertical axis in Line Graph?

I want to add tertiary vertical axis in line graph as seen in attached picture

04 May 2017 4,595 4 View

Does Journal Impact Factor Really Matters when getting PhD?

31 December 2016 7,904 6 View

Can I get Gene accession number by submitting single strand 16S rDNA sequence to NCBI or Double strand 16S rDNA sequence is required for this purpose?

Can I get Gene accession number by submitting single strand 16S rDNA sequence of bacteria to NCBI or Double strand 16S rDNA sequence is required for this purpose?

31 December 2016 10,137 6 View

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree?

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree

31 December 2016 5,022 9 View

How to select 1% bacterial culture for inoculation purpose?

Read 6 answers by scientists with 6 recommendations from their colleagues to the question asked by Muhammad Saleem Kalhoro on May 5, 2016

04 May 2016 8,387 7 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Surajvanshikumar Suvarna

You can store it for 1-2 months. Try storing it at room temp. Refrigeration temp. can cause salts to precipitate.

Jimin Yuan

No bacteria, no problem.

Pairat Sornplang

New prepared PBS or medium solution can be kept longer up under refrigerator temperature. And should be used within 1 week.