Muhammad Saleem Kalhoro

19 Questions 35 Answers 0 Followers

Questions related from Muhammad Saleem Kalhoro

Can we judge the quality of scientific journal by its impact factor alone?

What are other good indicators of quality journal apart from high impact factor

11 November 2018 7,915 24 View

How to prepare bacterial culture for Scanning Electron Microscope (SEM) study?

I want to prepare lactic acid bacteria from SEM study

01 January 2018 2,256 3 View

How to save Excel graph as image with 300 DPI?

I tried both MS paint and MS word, then saved as image but DPI is 96, conversion from 96 to 300 DPI through software dose not improve image quality, I am looking for direct method to save Excel...

09 September 2017 1,797 43 View

How to prepare 20 mM sodium acetate buffer (pH 4.6)?

How to prepare 20 mM sodium acetate buffer (pH 4.6)

07 July 2017 8,364 4 View

How to add Tertiary Vertical axis in Line Graph?

I want to add tertiary vertical axis in line graph as seen in attached picture

05 May 2017 4,467 4 View

How to prepare 100 ml HCL-KCL 10 mM buffer?

How to prepare 100 ml HCL-KCL 10 mM buffer, pH 4.6Will there be a change in pH after autoclave?

05 May 2017 9,236 2 View

Does Journal Impact Factor Really Matters when getting PhD?

01 January 2017 7,850 6 View

Can I get Gene accession number by submitting single strand 16S rDNA sequence to NCBI or Double strand 16S rDNA sequence is required for this purpose?

Can I get Gene accession number by submitting single strand 16S rDNA sequence of bacteria to NCBI or Double strand 16S rDNA sequence is required for this purpose?

01 January 2017 10,081 6 View

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree?

How to submit single strand 16s rDNA gene sequence to NCBI and create phylogenetic tree

01 January 2017 4,947 9 View

How long prepared PBS 7.0 (Phosphate Buffered Saline) can be stored?

PBS 7.2 (Himedia) adjusted to pH 7.0 stored at 4-8 °C in refrigerator

10 October 2016 4,277 3 View

How to differentiate/ identify Lactobacilli species without molecular methods?

I wanted to know about detection tests beside API or molecular techniques which can differentiate lactobacilli species

05 May 2016 3,371 8 View

What is the difference between Single strand 16S rDNA sequencing and Double strand 16S rDNA sequencing?

Can I use Single strand 16S rDNA sequencing to identify lactic acid bacteria up to species level with similarity percentage?

05 May 2016 6,902 3 View

What is the difference between 16S rRNA sequencing and 16S rDNA sequencing?

Which one from them should be used for identification of Lactic acid bacteria

05 May 2016 4,768 3 View

How to select 1% bacterial culture for inoculation purpose?

Read 6 answers by scientists with 6 recommendations from their colleagues to the question asked by Muhammad Saleem Kalhoro on May 5, 2016

05 May 2016 8,325 7 View

How to prepare 150 mM NaHCO3 (Sodium Bicarbonate)?

02 February 2016 1,049 6 View

Why are Lactic acid bacteria cultured anaerobically?

Lactic acid bacteria are facultative bacteria capable of growing in presence or absence of oxygen but in most of the research papers I read, they are cultured anaerobically

01 January 2016 278 5 View

Which one better for the serial dilution of lactic acid bacteria, saline or peptone water?

Which one is better for the serial dilution of lactic acid bacteria, saline or peptone water

12 December 2015 3,629 4 View

Is it okay to use laminar air flow blower for drying agar plates?

How long agar plates should be left to dry?

12 December 2015 4,408 1 View

How to prepare MRS agar slants?

How to prepare MRS agar slants? How long will lactic acid bacteria colonies be preserved on MRS agar slants and what should be the storage temperature?

12 December 2015 2,655 4 View