I have a 0.2 M sodium phosphate buffer (pH 7.4) with such composition: Water 97.17%, sodium phosphate dibasic 2.75%, and sodium phosphate monobasic 0.08%.
How can I prepare 50 mM sodium phosphate buffer (pH 6.5)?
Dear Berzhan,
In a simple way, you can dilute the stock solution in 4 times with deionized water. Then for adjustment of pH in 6.5, phosphoric acid is better than other acids because your buffer is based on sodium phosphate.
Sincerely yours, Homayoon.
Not the best way to do it, better get NaH2PO4 and Na2HPO4, prepare 50 mM solutions of both and mix those in a ratio calculated from the buffer equation.
If that can't be done, add acid to your buffer to adjust the pH (using a pH-meter) and then dilute 4-fold (that is, you'll need 25 ml of the original buffer for 100 mL final solution).
Dear Berzhan,
In a simple way, you can dilute the stock solution in 4 times with deionized water. Then for adjustment of pH in 6.5, phosphoric acid is better than other acids because your buffer is based on sodium phosphate.
Sincerely yours, Homayoon.
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