Berzhan Kurmanov

5 Questions 3 Answers 0 Followers

Questions related from Berzhan Kurmanov

How to use BLAST to find all the sequences in a genome that are located between two given sequences?

For example, I need to find all sequences flanked with two sequences CAGGAACGCTACC and TGCGCTTCGACAG: CAGGAACGCTACC...NNNNN...TGCGCTTCGAC, where ...NNNNN... means any sequence with any size, or...

28 April 2022 8,267 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

26 February 2021 8,424 3 View

Problem with Metaphor gel preparation. What could be the reason?

I am trying to prepare a Metaphor agarose gel, but after about 20 minutes at room temperature and 20 minutes at +4 C it is still not solid and when I try to remove the comb it falls apart. What...

18 August 2020 7,597 0 View

How to determine a plasmid copy number using qPCR?

For example we have a bacterium containing two plasmids, one of which carries a target gene. Is it possible to calculate the number of copies of this particular plasmid performing real-time PCR...

18 November 2019 3,487 3 View

How to prepare 50 mM sodium phosphate buffer (pH 6.5) from a 0.2 M stock solution (pH 7.4)?

I have a 0.2 M sodium phosphate buffer (pH 7.4) with such composition: Water 97.17%, sodium phosphate dibasic 2.75%, and sodium phosphate monobasic 0.08%. How can I prepare 50 mM sodium...

18 February 2019 2,458 4 View