Prepare sufficient 0.1 M EDTA stock solution. Take aliquots of it to dilute it to 1/2, 1/5, 1/20 and 1/100 of the original strength with distilled water. Then you have your required solutions.

Dear  Natarajan,

if you come from other fields of research not necessary well linked to chemistry, you can find here useful links for solution preparation:

http://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/mass-molarity-calculator.html

http://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/solution-dilution-calculator.html

dear Karthika,

there are two ways you can prepare EDTA solution of different concentration.

1. by preparing the higher concentration of stock solution and subsequently diluting in solvent system. (normally EDTA dissolves in alkaline pH, or by adding EDTA to a slightly warmed distilled water)

2. By  directly dissolving: required calculated mass (g) of EDTA in the solvent system.

For this:  the Molecular weight of EDTA, Volume required, Morality.

Mass (g)   =  (M.Wt of EDTA * Molarity * vol required) / 1000

you can use below link for calculation:

http://www.graphpad.com/quickcalcs/Molarityform.cfm

I agree with above. Thanks.

Take care. Solid edta is not always 100% pure.

Prepare the solutions as indicated before. But titrate it against calcium each month .

I have found some degradation with time.

thank you everyone...

The suggestion made by Bhavya G  is in order.Thanks

The suggestion made by Bhavya is very useful. Make sure that you test tube are properly clean devoid of any impurities

Thank you so very much

This thread helped me as well. Thank you!

Thanks alot for the information

The information was useful to me as well. Thank you everyone.

To prepare 0.01 M , dissolve 3.7 gm EDTA in 1 L water