Why is selected ion monitoring more selective and sensitive?

Why is selected ion monitoring more selective and sensitive compared to SCAN MODE?? I think scan seems better than sim somewhat unconditionally.

03 April 2016 10,014 6 View

Phylogenetically speaking, why can human insulin be used to treat dog diabetes?

Phylogenetically speaking, why can human insulin be used to treat dog diabetes?

02 March 2016 108 1 View

Where do I get the detailed, easy-to-understand principle for solid next generation sequencing?

Where do I get the detailed, easy-to-understand principle for solid next generation sequencing?

02 March 2016 1,432 3 View

How do you solve this differential equation used for quadrupole in Mass spectrometer?

Here there are three equations, two being the differential equations, and the third one is the solution. I would like to know how to reach this solution.

02 March 2016 9,532 2 View

How to solve my blast problem?

02 March 2016 3,721 3 View

How to find the sequence for CpG sites?

02 March 2016 8,234 9 View

Is thin layer chromatography still used in medicine companies?

02 March 2016 9,958 3 View

Disadvantages of using sequence specific primers?

I know that there are two disadvantages of using sequence specific primers. I do not quite understand the disadvantages. First one is "only one assay can be run from each cDNA reaction." I am not...

01 February 2016 8,529 5 View

How is uracil changed to thymine after bisulfite treatment?

after bisulfite treatment, unmethylated cytosine is changed to uracil. How is the uracil converted to thymine??

01 February 2016 5,288 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View