Bisulfite specific primers for these three sites are included in these supplemental materials:

https://static-content.springer.com/esm/art%3A10.1186%2Fgb-2014-15-2-r24/MediaObjects/13059_2013_3218_MOESM1_ESM.pdf

to this paper:

http://genomebiology.biomedcentral.com/articles/10.1186/gb-2014-15-2-r24#CR41

The primers can be used for bisulfite specific in silico PCR at this site:

http://bisearch.enzim.hu/?m=genompsearch

For the ASPA primers, the result returned is this:

PCR product(s) on the bisulfite transformed sense chain

Forward primer: ATTATTTGGTGAAATGATT

Reverse primer: CAACCCTATTCTCTAAATCTC

1. Chromosome 17 (len: 151)

3476146 ATTATTTGG TGAAATGATT TTTTGTTATA TTGTTGAAGA ATATATATAA

AAGGTTGTTA TTTTTGGAGG AATTTATGGG AATGAGTTAA TTGGAGTATT

TTTGGTTAAG TATTGGTTAG AGAATGGTGT TGAGATTTAG AGAATAGGGT

TG 3476297

The coordinates shown are for genome build:Homo sapiens GRch 38.83.

You can obtain the "normal" genomic sequence by going to the genome browser gateway,

http://genome.ucsc.edu/cgi-bin/hgGateway

Select human genome build 38 and entering the coordinates shown in the format chr71:3,476,146-3,476,297 into the search box.....returning:

http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr17%3A3476146-3476297&hgsid=480626633_SpZtxmzaIS5afMfhpVpjI6GcZ2W7

They are CpG IDs from Illumina's methylation array. You should be able to find the position and sequence on Illumina's website.

Bisulfite specific primers for these three sites are included in these supplemental materials:

https://static-content.springer.com/esm/art%3A10.1186%2Fgb-2014-15-2-r24/MediaObjects/13059_2013_3218_MOESM1_ESM.pdf

to this paper:

http://genomebiology.biomedcentral.com/articles/10.1186/gb-2014-15-2-r24#CR41

The primers can be used for bisulfite specific in silico PCR at this site:

http://bisearch.enzim.hu/?m=genompsearch

For the ASPA primers, the result returned is this:

PCR product(s) on the bisulfite transformed sense chain

Forward primer: ATTATTTGGTGAAATGATT

Reverse primer: CAACCCTATTCTCTAAATCTC

1. Chromosome 17 (len: 151)

3476146 ATTATTTGG TGAAATGATT TTTTGTTATA TTGTTGAAGA ATATATATAA

AAGGTTGTTA TTTTTGGAGG AATTTATGGG AATGAGTTAA TTGGAGTATT

TTTGGTTAAG TATTGGTTAG AGAATGGTGT TGAGATTTAG AGAATAGGGT

TG 3476297

The coordinates shown are for genome build:Homo sapiens GRch 38.83.

You can obtain the "normal" genomic sequence by going to the genome browser gateway,

http://genome.ucsc.edu/cgi-bin/hgGateway

Select human genome build 38 and entering the coordinates shown in the format chr71:3,476,146-3,476,297 into the search box.....returning:

http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr17%3A3476146-3476297&hgsid=480626633_SpZtxmzaIS5afMfhpVpjI6GcZ2W7

Thank you for the answer...

What a relief after having got the answer so easily...

It was Too hard for me to work on it alone. Really appreciate this.

When I blasted the primer sequences in the paper in ncbi, I could not find ASPA as the result.

I guess here the primers primers for the sequence of ASPA gene after bisulfite treatment. Am I right on this part? If that is not the reason, what is the reason for not being able to detect ASPA after blast?

One more thing is this site http://bisearch.enzim.hu/?m=genompsearch is not working for PDE4C gene...do you know the reason for this ??

Anyways, I am very very very very grateful for your help. Really very very very.

@Chanwoo:

I guess here the primers primers for the sequence of ASPA gene after bisulfite treatment. Am I right on this part?

YES!

One more thing is this site http://bisearch.enzim.hu/?m=genompsearch is not working for PDE4C gene...do you know the reason for this ??

This primer pair does work.  (My earlier version of this post was in error!)

PCR product(s) on the bisulfite transformed antisense chain

Forward primer: AGGTTTGTAGTAGGTTGA

Reverse primer: AACTCAAATCCCTCTC

1. Chromosome 19 (len: 156)

18233004 AACTCA AATCCCTCTC

ACAACAACCC ACAAACTTAT CCAAATCCAA ATAAAAACAC TATTAAATAC

AAATAAAACA CCAAAATTAC CACCACAAAT ACTTCAAAAC TCTAATCATA

CTATAACAAC CACAAAAACA ACTCAACCTA CTACAAACCT 18233160

And these coordinates do correspond to the PDE4C promoter/CpG island.

http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&lastVirtModeType=default&lastVirtModeExtraState=&virtModeType=default&virtMode=0&nonVirtPosition=&position=chr19%3A18233004-18233160&hgsid=480699137_esrBX1KM7kB5jytAq8drCzx5FAcN

upvoted for an excellent concise answer, thank you David f Barker

Regarding PDE4C: published primer sequence of reverse PCR primer seems to be correct, doesn't understand Davids remark on 3'C??

5 ́-AACTCAAATCCCTCTC-3 ́, that's a well working reverse primer for above PCR of PDE4C.

@Uwe.  Thanks for pointing that out!  I had a cut and paste mishap!  I've corrected my earlier post.

I thing the question was how does one locate the sequence cg02228185 in ASPA.  This CpG site not in the gene itself which is on human chromosome 17. Perhaps this is corresponding to an upstream promoter region or cis acting elelment?