Why is selected ion monitoring more selective and sensitive?

Why is selected ion monitoring more selective and sensitive compared to SCAN MODE?? I think scan seems better than sim somewhat unconditionally.

03 April 2016 10,014 6 View

Where do I get the detailed, easy-to-understand principle for solid next generation sequencing?

Where do I get the detailed, easy-to-understand principle for solid next generation sequencing?

02 March 2016 1,432 3 View

How do you solve this differential equation used for quadrupole in Mass spectrometer?

Here there are three equations, two being the differential equations, and the third one is the solution. I would like to know how to reach this solution.

02 March 2016 9,532 2 View

How to observe dna contamination in RNA using gel electrophoresis?

How to observe dna contamination in RNA using gel electrophoresis?

02 March 2016 3,606 2 View

How to solve my blast problem?

02 March 2016 3,721 3 View

How to find the sequence for CpG sites?

02 March 2016 8,234 9 View

Is thin layer chromatography still used in medicine companies?

02 March 2016 9,958 3 View

Disadvantages of using sequence specific primers?

I know that there are two disadvantages of using sequence specific primers. I do not quite understand the disadvantages. First one is "only one assay can be run from each cDNA reaction." I am not...

01 February 2016 8,529 5 View

How is uracil changed to thymine after bisulfite treatment?

after bisulfite treatment, unmethylated cytosine is changed to uracil. How is the uracil converted to thymine??

01 February 2016 5,288 3 View

How can the use of Jolly Phonics improves the quality of reading, speaking and writing in rural disadvantaged communities in Papua New Guinea?

Jolly Phonics reading program is an effective way to teach early reading to young learners. How can lectures at the university prepare early childhood student teachers to teach Jolly Phonics to...

03 March 2021 3,731 3 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Do I need to introduce HFD to ob/ob mice to obtain a T2DM model?

I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...

18 February 2021 6,896 3 View

Does the phylogenetic tree making tool IQTREE use evolutionary placement algorithm?

For my master thesis I am conducting a phylogenetic research and for this I'm using tools like MEGA, IQTREE, FigTree, etc to make a phylogenetic tree. I wanted to know if IQTREE integrates the...

14 February 2021 1,541 2 View

Can anyone suggest a way to dissolve insulin which is for rat/mouse injection?

We are trying to inject insulin to rat or mouse, but the insulin we've got is powder, and there are many ways for dissolve, like in saline, PBS, DMSO, or in Tris-HCl, can anyone give us a...

13 February 2021 8,426 10 View

Inter-rater / inter-coder reliability for thematic analysis?

In my research, I have used metaphorical elicitation tasks to investigate learners' conceptualization of speaking fluency. In the analysis phase, I used thematic analysis to make themes out of...

10 February 2021 4,719 11 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Bradford assay for insulin: discrepancies between standard curve and sample concentration during crystallisation?

Hi there, I would like to determine the unknown concentration of human insulin in my sample during crystallisation (the concentration range is roughly 0.5 -2 mg/ml, whereas the initial...

06 February 2021 9,370 7 View

Is there a protocol for the insulin encapsulation at physiological pH?

Hello everyone, I am going to start my master thesis on the encapsulation of insulin. In almost all protocols we found an acidic pH is necessary. Unfortunately, pH

27 January 2021 3,692 5 View