Is the supervisor wrong if he helped his student to complete part of his thesis?

Sometimes I help my students meet some of the requirements of their studies to feel the spirit of cooperation in accomplishing the work.

02 March 2019 4,881 3 View

How can gibberellin be added to avoid heat damage during medium sterilization by autoclave?

Medium sterilization.

10 November 2017 9,264 0 View

Is there a protocol for the propagation of eggplant plant in the somatic embryo genesis method?

Micro propagation of Solanum melongena

10 November 2017 2,390 2 View

Can someone give me any research on the production of happier summer squash plants?

In vitro propagation.

10 November 2017 6,622 1 View

What are the active substances s in Morinag plant?

Morninga as medicinal plant.

09 October 2017 9,120 3 View

What are the best waxes used in fruit coating ?

Anti-evaporation of water

09 October 2017 2,252 3 View

No title

Flowering in fruit trees.

05 June 2017 539 2 View

Why lipid peroxidation increased with increasing the salt concentrations ?

Plant cells in salt stress.

05 June 2017 857 3 View

Can anyone give me the best method to lipid peroxidation determination ?

Salt stress experiment by in vitro culture.

05 June 2017 6,429 12 View

What's the role of pvp in in vitro culture technique?

Pvp added to white medium.

04 May 2017 8,375 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

No title

17 February 2021 4,217 3 View

How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View