Is the supervisor wrong if he helped his student to complete part of his thesis?

Sometimes I help my students meet some of the requirements of their studies to feel the spirit of cooperation in accomplishing the work.

02 March 2019 4,881 3 View

How can gibberellin be added to avoid heat damage during medium sterilization by autoclave?

Medium sterilization.

10 November 2017 9,264 0 View

Is there a protocol for the propagation of eggplant plant in the somatic embryo genesis method?

Micro propagation of Solanum melongena

10 November 2017 2,390 2 View

Can someone give me any research on the production of happier summer squash plants?

In vitro propagation.

10 November 2017 6,622 1 View

What are the active substances s in Morinag plant?

Morninga as medicinal plant.

09 October 2017 9,120 3 View

What are the best waxes used in fruit coating ?

Anti-evaporation of water

09 October 2017 2,252 3 View

No title

Flowering in fruit trees.

05 June 2017 539 2 View

Can anyone give me the best method to lipid peroxidation determination ?

Salt stress experiment by in vitro culture.

05 June 2017 6,429 12 View

What's the role of pvp in in vitro culture technique?

Pvp added to white medium.

04 May 2017 8,375 0 View

Anyone give me some information about Arabidopsis?

04 May 2017 3,194 0 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to modify micro-structure of Pure Copper which is available as a flat plate of 25 X 25 sq. cm?

Hi everyone, for a thesis project I have to modify the micro-structural properties of pure Cu flat plate of dimension 25 X 25 sq. cm . As I tried searching the available literature, I found a lot...

09 February 2021 5,090 3 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

References for relative humidity created by salt solutions at different temperatures?

I understand that the ability of standard salt solutions commonly used to produce a certain relative humidity (RH) in a closed space changes somewhat with the temperature. Where do I find data...

04 February 2021 7,370 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View