This is a very good method.
Potter TM, Neun BW, Stern ST. Assay to detect lipid peroxidation upon exposure to nanoparticles. Methods Mol Biol. 2011;697:181-9
Dear Majid Abdulhameed Ibrahim
I attached files for you "Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction". The method have 20133 citation.
Also I have attached other file about LPo.
Thiobarbituric acid assay for measuring lipid oxidation
http://cdn.intechopen.com/pdfs/38473/InTech-Automation_of_methods_for_determination_of_lipid_peroxidation.pdf
https://core.ac.uk/download/pdf/11616188.pdf
http://pubs.acs.org/doi/abs/10.1021/jf00021a018?journalCode=jafcau
http://s3.amazonaws.com/academia.edu.documents/45657132/The_effect_of_salt_stress_on_lipid_perox20160516-18874-1fvkrdb.pdf?AWSAccessKeyId=AKIAIWOWYYGZ2Y53UL3A&Expires=1497279844&Signature=mHah5TMAuFcqKBofEQbfHa5
I suggest you , MDA measurement is The most accurate method to detrminate the lipid peroxidation
Malondialdehyde (MDA) is one of the final products of polyunsaturated fatty acids peroxidation in the cells. An increase in free radicals causes overproduction of MDA. Malondialdehyde level is commonly known as a marker of determination of lipid peroxidation
Methods based on MDA level determination are accurate precise and easy to handle..
Yes, MDA measurement is commonly used method for Lipid Peroxidation.
MDA measures technique is mostly used for lipid peroxidation and oxidative stress end product
Thesis Correlation of Leptin and Resistin with Oxidative Stress in ...
Another method you could use is that of fluorescent lipid products. It's very simple and when we can not do TBARS we use this one. It is'n the best but is an option
8-iso-PGF2α (8-isoprostane) a stable prostaglandin-like product
formed from arachidonic acid by the direct action of ROS
Dear Majid
You can fallow below method:
1- 0.5 gr fresh tissue add to 5 ml TCA(0.1%) and then hemogenise it
2-then centrifuge it (15000 g for 10 min)
3-take 1 ml supernatant and add it to 4 ml TBA-TCA solution
TBA-TCA solution: 0.5 gr TBA in 20% TCA
4- incubate it for 30 min(in banmary)95 0C
5- then cool it in ice for 15 min(or refrigerator)
6-you can centrifuge it again(10000 g for 10 min)(if you like)
7-read absorbance at 532 nm and 600 nm
if you need help please inform me.
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