How to measure adsorption of biomolecules in liquid on solid surface?

More H.H Nguyen's questions See All

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Neuman Solange de Resende

FTIR is a powerful/useful analysis to evaluate the chemisorption on solid samples.

See J Am Chem Soc. 2003 Apr 30;125(17):5219-26.

Adam B Shapiro

If the adsorption results in a measurble decrease in the amount of free DNA in the solution, then you could separate the nanoparticles from the supernatant by centrifugation or filtration and measure the residual free DNA by its 260 nm UV absorbance. The amount adsorbed is the difference between the original supernatant concentration before the nanoparticles were added and the final supernatant concentration, times the volume.

H.H Nguyen

Thank you for your valueable answers Adam And Neuman.

Best Regards ~