H.H Nguyen

11 Questions 19 Answers 0 Followers

Questions related from H.H Nguyen

How temperature affect plasmid DNA conformation?

I conduct a thermal stability test for plasmid DNA. In which I put plasmid DNA under 65-degree celsius for about one week. After that, I run an agarose gel check. Here below is what I got: 3 left...

26 December 2016 8,907 3 View

What is the reason for the complete loss of transformation efficiency?

I have conducted a test on transformation efficiency of circular plasmid DNA after some harsh storage period (in which I put the plasmid DNA in 60 Celcius degree for 2 weeks). And results showed...

28 October 2016 1,904 4 View

What are the methods for nucleotide ratio quantitation?

Providing that I have a single stranded DNA (short fragment ~30 nt) with unknown sequence. What are the experimental methods which could help to determine nucleotide ratio (percentage of A,T,G,C)...

25 April 2016 7,283 2 View

How to measure adsorption of biomolecules in liquid on solid surface?

I would like to ask what techniques I could use to measure / monitor the adsorption of DNA on solid surfaces (In my case, it is metal nanoparticles)?

08 April 2016 4,018 3 View

What is the problem with my bacterial cell growth in LB media?

I am facing a weird situation where I got my ligated plasmid in DH5α growing well on the streak plate (overnight), but after I transfered the cells from the streak plate to LB medium, they grow...

15 March 2016 5,852 7 View

What universal primer should I choose for plasmid sequencing?

I ligated my insert into the between of Xho I and Nde I of pet28a vector (see image), and I am wonderfing if T7 promoter or T7 terminator should be chosen as the primer for sequencing. Could you...

17 December 2015 6,313 5 View

What is the minimum base-pairs required for DNA hybridization?

I am doing a DNA hybridization test and wondering how many base pairs i need at least for the hybridization reaction to occur. Could you please share your expertise on this. Thank you very much ~

28 May 2015 395 7 View

What is the proper washing and drying step for glass slide array?

I would like to ask about a correct washing and drying step for glass slide array experiment without the washing/hybridisation station from company. Washing: Is that as simple as using pipet to...

17 March 2015 6,350 1 View

Could an incorrect dilution affect the reaction outcome?

I think there is a difference between these 2 dillution methods and therefore it might affects the reaction (A+B=AB). Could you please give me some clear explainations on this: I have: Stock A: 10...

07 January 2015 8,098 3 View

EcoR I does not fully digest my DNA. Why?

I used a 80 bp ds DNA which contains EcoR I recognition site in the middle of the strain, my objective is to obtain 40 bp ds DNA fragment. I used the protocol supplied with my enzyme (from...

04 August 2014 6,216 7 View

What is the form of synthesized oligonucleotides, A or B?

Native DNA in cells is known to be in B form, mostly. What about synthesized one?

09 June 2014 1,658 2 View