Dear researchers,
How to make elution buffer for SDS-PAGE?
There is a lot of protocol to make elution buffer, but they didn't show the amount of each ingredient needed.
thank you.
Jennifer Leong , may be are you looking for it?
http://microbiology.ucdavis.edu/privalsky/sites/default/files/SDS-PAGE_0.pdf -available all solution for SDS-PAGE
10 x SDS-PAGE Running Buffer
l30.3 g Tris base
144.0 g Glycine
10.0 g SDS 10
Dissolve and bring total volume to 1 L deionized water. Do not adjust pH with acid or base (pH is normally 8.3 as prepared).
Are you talking about running buffer?
Andrea Brenna
Nope, I am talking about the elution buffer which is used in the protein elution from SDS PAGE.
There are many different elution buffers. It depends from the experiment you are performing. Can you tell me what are you exactly doing?
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